Differential display by PCR: novel findings and applications
نویسندگان
چکیده
منابع مشابه
Differential Display-PCR.
This method uses PCR to amplify and display many cDNAs derived from the mRNAs of a given cell or tissue type. The method relies on two different types of synthetic oligonucleotides: anchored antisense primers and arbitrary sense primers. A typical anchored primer is complementary to approx. 13 nucleotides of the poly(A) tail of mRNA and the adjacent two nucleotides of the transcribed sequence. ...
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The polymerase chain reaction (PCR)-based mRNA differential display (3) has been widely used for identifying differentially expressed mRNA in a variety of biological systems (4). mRNA differential display consists of two basic steps: (i) reverse transcription (RT) using a set of 3′-anchored primers, T12MN (M = G, A or C; N = G, A, T or C) and (ii) PCR amplification of cDNA fragments using an ar...
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~The first two authors contributed equally to the establishment of the method and to the results obtained. 4present address: Max-Planck-Gesellschaft, MaxDelbr~ick-Haus, 13122 Berlin-Buch, Germany. Analysis of gene expression is a central aim in most studies in molecular and cell biology. Interest in tissue-specific gene expression and, in particular, in changes in the expression patterns occurr...
متن کاملDifferential expression of COUP-TFI, CHL1, and two novel genes in developing neocortex identified by differential display PCR.
Genes that control the specification and differentiation of the functionally specialized areas of the mammalian neocortex are likely expressed across the developing neocortex in graded or restricted patterns. To search for such genes we have performed a PCR-based differential display screen using RNAs from rostral neocortex, which included the primary motor area, and caudal neocortex, which inc...
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The host-fungus interaction is characterized by changes in gene expression in both host and pathogen. Differential-display reverse transcription PCR (DDRT-PCR) is a PCR-based method that allows extensive analysis of gene expression among several cell populations. Several limitations and drawbacks to this procedure have now been addressed, including the large number of false-positive results and...
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ژورنال
عنوان ژورنال: Nucleic Acids Research
سال: 1995
ISSN: 0305-1048,1362-4962
DOI: 10.1093/nar/23.10.1832